全文获取类型
收费全文 | 35671篇 |
免费 | 16954篇 |
国内免费 | 6810篇 |
出版年
2024年 | 73篇 |
2023年 | 367篇 |
2022年 | 793篇 |
2021年 | 1047篇 |
2020年 | 2850篇 |
2019年 | 4445篇 |
2018年 | 4264篇 |
2017年 | 4606篇 |
2016年 | 4569篇 |
2015年 | 4631篇 |
2014年 | 4472篇 |
2013年 | 4809篇 |
2012年 | 2903篇 |
2011年 | 2495篇 |
2010年 | 3892篇 |
2009年 | 2757篇 |
2008年 | 1703篇 |
2007年 | 1296篇 |
2006年 | 1215篇 |
2005年 | 1112篇 |
2004年 | 870篇 |
2003年 | 754篇 |
2002年 | 718篇 |
2001年 | 744篇 |
2000年 | 618篇 |
1999年 | 396篇 |
1998年 | 145篇 |
1997年 | 97篇 |
1996年 | 77篇 |
1995年 | 66篇 |
1994年 | 64篇 |
1993年 | 52篇 |
1992年 | 58篇 |
1991年 | 40篇 |
1990年 | 33篇 |
1989年 | 40篇 |
1988年 | 35篇 |
1987年 | 31篇 |
1986年 | 38篇 |
1985年 | 32篇 |
1984年 | 25篇 |
1983年 | 16篇 |
1982年 | 32篇 |
1981年 | 16篇 |
1979年 | 9篇 |
1978年 | 11篇 |
1959年 | 12篇 |
1957年 | 11篇 |
1954年 | 9篇 |
1951年 | 7篇 |
排序方式: 共有10000条查询结果,搜索用时 62 毫秒
51.
P. Cruz C. H. Mejia‐Ruiz R. Perez‐Enriquez A. M. Ibarra 《Molecular ecology resources》2002,2(3):239-241
Five polymorphic microsatellite loci were characterized for Penaeus (Litopenaeus) vannamei. Loci were isolated using a partial Sau3A1 genomic library by the sequencing of randomly selected clones and by a biotinylated (CT)10 and (GT)10 probes screening procedure. The last strategy resulted in the most useful data. About 40% of the clones showed a previously reported satellite/microsatellite (PVS1), reducing the chance of finding new microsatellite regions. Whereas two of the microsatellite loci with more than 10 alleles will be useful for mating analysis in a breeding program, the others might prove useful for population genetic studies. 相似文献
52.
53.
54.
55.
56.
57.
T. Phanitchat C. Apiwathnasorn S. Sungvornyothin Y. Samung S. Dujardin J.‐P. Dujardin S. Sumruayphol 《Medical and veterinary entomology》2019,33(4):476-484
Wing geometry helps to identify mosquito species, even cryptic ones. On the other hand, temperature has a well‐known effect on insect metric properties. Can such effects blur the taxonomic signal embedded in the wing? Two strains of Aedes albopictus (laboratory and field strain) were examined under three different rearing temperatures (26, 30 and 33 °C) using landmark‐ and outline‐based morphometric approaches. The wings of each experimental line were compared with Aedes aegypti. Both approaches indicated similar associations between wing size and temperature. For the laboratory strain, the wing size significantly decreased as the temperature increased. For the field strain, the largest wings were observed at the intermediate temperature. The two morphometric approaches describing shape showed different sensibilities to temperature. For both strains and sexes, the landmark‐based approach disclosed significant wing shape changes with temperature changes. The outline‐based approach showed lesser effects, detecting significant changes only in laboratory females and in field males. Despite the size and shape changes induced by temperature, the two strains of Ae. albopictus were always distinguished from Ae. aegypti. The present study confirms the lability of size. However, it also suggests that, despite environmentally‐induced variation, the architecture of the wing still provides a strong taxonomic signal. 相似文献
58.
Lauren Petrullo Matthew J. Jorgensen Noah Snyder‐Mackler Amy Lu 《American journal of primatology》2019,81(10-11)
The human milk microbiome is vertically transmitted to offspring during the postnatal period and has emerged as a critical driver of infant immune and metabolic development. Despite this importance in humans, the milk microbiome of nonhuman primates remains largely unexplored. This dearth of comparative work precludes our ability to understand how species‐specific differences in the milk microbiome may differentially drive maternal effects and limits how translational models can be used to understand the role of vertically transmitted milk microbes in human development. Here, we present the first culture‐independent data on the milk microbiome of a nonhuman primate. We collected milk and matched fecal microbiome samples at early and late lactation from a cohort of captive lactating vervet monkeys (N = 15). We found that, similar to humans, the vervet monkey milk microbiome comprises a shared community of taxa that are universally present across individuals. However, unlike in humans, this shared community is dominated by the genera Lactobacillus, Bacteroides, and Prevotella. We also found that, in contrast to previous culture‐dependent studies in humans, the vervet milk microbiome exhibits greater alpha‐diversity than the gut microbiome across lactation. Finally, we did not find support for the translocation of microbes from the gut to the mammary gland within females (i.e., “entero‐mammary pathway”). Taken together, our results show that the vervet monkey milk microbiome is taxonomically diverse, distinct from the gut microbiome, and largely stable. These findings demonstrate that the milk microbiome is a unique substrate that may selectively favor the establishment and persistence of particular microbes across lactation and highlights the need for future experimental studies on the origin of microbes in milk. 相似文献
59.
60.
为研究青藏高原草地承载力的空间演变特征并对其进行预警,以已有的青藏高原净初级生产力数据为基础,核算了该地区的草地理论载畜量及演变趋势,并结合县域实际存栏量,划定了草地承载力的预警等级。结果表明:(1)青藏高原草地承载力整体呈东高西低的格局,其中高寒草原和高寒草甸是该地区草地承载力的主要组成部分;(2)2000-2015年,青藏高原理论载畜量由8614.89万羊单位增至9451.53万羊单位;(3)青藏高原整体处于超载状态,2000-2010年超载状况加剧,至2015年超载状况稍有缓解,草畜平衡指数由67.88%增至79.90%,再降至67.91%。目前亟需优先控制72个红色预警县(超载状态正在加剧)的牲畜存栏量,避免超载状况进一步恶化。未来需要通过控制牲畜存栏量、调整畜牧区发展布局和提高草地生产力等多项措施的结合来改善青藏高原地区的草地承载状况,维持草地生态系统的可持续发展。 相似文献